Electron microscopy of Kinesin-Microtubule complexes
Motorhome

By Keiko Hirose and Linda Amos
[Hirose, K., Lockhart, A. Cross, R.A. and Amos,L.A. (1996) Three dimensional cryoelectron microscopy of dimeric kinesin and ncd motor domains on microtubules PNAS 93 9539-9544]

These image reconstructions are from electron micrographs of rapidly frozen hydrated specimens. Local mirror of kinesin homepage article by Amos Hirose and Cross.

Click the images to see a full size version.

	Undecorated 16PF Microtubule.
	Microtubule decorated with single rat kinesin heads.
	Microtubule decorated with K430, recombinant 2-headed rat kinesin.
	Microtubule decorated with GSTMC5, recombinant 2-headed drosophila ncd.
	Montage
	Montage from earlier negative stain images [Hirose, K., Lockhart, A., Cross, R.A. & Amos, L.A. (1995) Nucleotide-dependent angular change in kinesin motor domain bound to tubulin Nature 376 277-279 ] showing conformational shifts associated with a change of nucleotide occupant of the active site. Animation.
	Opened out tubulin sheets decorated with [left panels] single kinesin heads and [right 2 panels] single ncd heads.